Journal: bioRxiv

Article Title: Signatures of divergent antimalarial treatment responses in peripheral blood from infants and adults in Malawi

doi: 10.1101/564757

Figure Lengend Snippet: (A) The test result proportions for overall circulating antimalarial IgG and IgM, measured by ELISA test of blood samples, are shown. The change in test result status between visit 1 and 2 is shown for (B) infants and (C) adults. Colors indicate positive (red), uncertain (grey), and negative (yellow) test results.

Article Snippet: We assessed antimalarial antibodies using a semi-quantitative human malaria antibody ELISA kit (IBL International Inc., Hamburg, Germany #RE58901), according to manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) SARS-CoV-2 titers measured by plaque assay 3 days after sample exposure to each UV 222 dose (dark circles) were fit with an exponential model starting at the mean initial (0 mJ/cm ) viral titer of 6.51×10 4 PFU/mL through responses up to and including 8 mJ/cm where PFU/mL first dropped below the assay detection limit (DL) of 2 PFU/mL (hollow circles). Error bars represent standard deviation of at least two technical replicates. (B) SARS-CoV-2 log 10 reductions (LR) of viral titers after exposure to each UV 222 dose (dark circles) were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 8 mJ/cm where LR first exceeded the DL of 4.51 logs (hollow circles).

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: Plaque Assay, Standard Deviation

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3) expressed as log 10 reduction of N1 (short amplicon) copies/µL in qPCR reactions. (B) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) expressed as log 10 reduction of N1-2 (long amplicon) copies/µL in qPCR reactions. (C) SARS-CoV-2 N protein concentration measured by ELISA expressed as log 10 reduction of N protein concentration (pg/mL) in samples immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3). SARS-CoV-2 log 10 reductions of the N1 amplicon, N1-2 amplicon, or N protein versus UV 222 dose were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 20 mJ/cm indicated by filled circles. Points not included in models are indicated by hollow circles.

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: Incubation, Amplification, Protein Concentration, Enzyme-linked Immunosorbent Assay

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) The raw spectral emission from 200 - 300 nm of the filtered excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations and plotted on log scale to show orders of magnitude less but non-zero emission at filtered wavelengths > 240 nm. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three Tests for use in UV dose calculations.

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Rapid Detection Strategies for the Global Threat of Zika Virus: Current State, New Hypotheses, and Limitations

doi: 10.3389/fmicb.2016.01685

Figure Lengend Snippet: Methods available and applied for Zika virus detection.

Article Snippet: ZIKV IgG or IgM ELISA kits based on utilizing a double-antigen sandwich ELISA will be introduced by MyBiosource in USA.

Techniques: Virus, Next-Generation Sequencing, DNA Synthesis, Recombinant, Amplification, Multiplex Assay, Genomic Sequencing, Cell Culture, Control, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Reverse Transcription, Detection Assay

Journal: Frontiers in Microbiology

Article Title: Rapid Detection Strategies for the Global Threat of Zika Virus: Current State, New Hypotheses, and Limitations

doi: 10.3389/fmicb.2016.01685

Figure Lengend Snippet: Update on commercially available detection kits for Zika virus.

Article Snippet: ZIKV IgG or IgM ELISA kits based on utilizing a double-antigen sandwich ELISA will be introduced by MyBiosource in USA.

Techniques: Virus, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Real-time Polymerase Chain Reaction, Multiplex Assay, Reverse Transcription, Polymerase Chain Reaction